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Can I use another brand of T4 ligase?
Can I use another brand of T4 ligase?
For customers new to nanopore sequencing, we recommend buying the NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (catalogue number E7180).
We have validated and only recommend the use of all the third-party reagents specified in our protocols. We have not tested alternative T4 ligases.