No!

The V14 chemistry kits can only be run on R10.4.1 flow cells : FLO-FLG114 for Flongle, FLO-MIN114 for MinION / GridION and FLO-PRO114M for PromethION.

The legacy chemistry kits (prior to Kit V14 such as SQK-LSK114) can only be run on R9.4.1 flow cells. We have moved many of our low selling and previous chemistry sequencing kits to a new page on the store called Legacy. This ensures our users transition to our newest and best product line, while simplifying our product offering. For additional information we recommend customers to visit this page on our website https://nanoporetech.com/discontinued-kits