Direct RNA sequencing - sequence-specific (SQK-RNA002) (DSS_9081_v2_revT_25May2022)


DateVersionLanguageDeviceChanges
11/15/2023DSS_9081_v2_revT_25May2022EnglishGridION
November 2023 - Updated product phase to 'Legacy'
11/15/2023DSS_9081_v2_revT_25May2022EnglishMinION
November 2023 - Updated product phase to 'Legacy'
11/15/2023DSS_9081_v2_revT_25May2022EnglishPromethION
November 2023 - Updated product phase to 'Legacy'
September 19th, 2023DSS_9081_v2_revR_25May2022EnglishPromethion Download January 2023 - Update the priming and loading the flow cells for PromethION with new diagrams.
September 19th, 2023DSS_9081_v2_revR_25May2022EnglishGridion Download January 2023 - Update the priming and loading the flow cells for PromethION with new diagrams.
September 19th, 2023DSS_9081_v2_revR_25May2022EnglishMinion Download January 2023 - Update the priming and loading the flow cells for PromethION with new diagrams.
August 10th, 2022DSS_9081_v2_revQ_25May2022EnglishPromethion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022DSS_9081_v2_revQ_25May2022EnglishGridion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022DSS_9081_v2_revQ_25May2022EnglishMinion Download July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
May 25th, 2022DSS_9081_v2_revP_25May2022EnglishPromethion Download 25th May 2022 Have removed all references to poly(A)-tailed RNA use throughout the protocol, protocol now only references use of total RNA. Snippet was already present in Overview directing user to correct protocol for sequencing poly(A)-tailed RNA.
May 25th, 2022DSS_9081_v2_revP_25May2022EnglishGridion Download 25th May 2022 Have removed all references to poly(A)-tailed RNA use throughout the protocol, protocol now only references use of total RNA. Snippet was already present in Overview directing user to correct protocol for sequencing poly(A)-tailed RNA.
May 25th, 2022DSS_9081_v2_revP_25May2022EnglishMinion Download 25th May 2022 Have removed all references to poly(A)-tailed RNA use throughout the protocol, protocol now only references use of total RNA. Snippet was already present in Overview directing user to correct protocol for sequencing poly(A)-tailed RNA.
April 14th, 2022DSS_9081_v2_revO_14Aug2019EnglishPromethion Download 14th April 2022 Protocol name change
April 14th, 2022DSS_9081_v2_revO_14Aug2019EnglishGridion Download 14th April 2022 Protocol name change
April 14th, 2022DSS_9081_v2_revO_14Aug2019EnglishMinion Download 14th April 2022 Protocol name change
April 14th, 2022DSS_9081_v2_revO_14Aug2019EnglishAny Download 14th April 2022 Protocol name change
March 23rd, 2022DSS_9081_v2_revN_14Aug2019EnglishPromethion Download 23rd March 2022 - Updated input in the introduction of the protocol - Added further information regarding RNA input in the equipment and consumables section - Updated SPRI clean volume in the library prep
March 23rd, 2022DSS_9081_v2_revN_14Aug2019EnglishGridion Download 23rd March 2022 - Updated input in the introduction of the protocol - Added further information regarding RNA input in the equipment and consumables section - Updated SPRI clean volume in the library prep
March 23rd, 2022DSS_9081_v2_revN_14Aug2019EnglishMinion Download 23rd March 2022 - Updated input in the introduction of the protocol - Added further information regarding RNA input in the equipment and consumables section - Updated SPRI clean volume in the library prep
September 13th, 2021DSS_9081_v2_revM_14Aug2019EnglishPromethion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021DSS_9081_v2_revM_14Aug2019EnglishGridion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
September 13th, 2021DSS_9081_v2_revM_14Aug2019EnglishMinion Download 13th Sept 2021 - Added table outlining kit contents in the Equipment and Consumables section.
June 10th, 2021DSS_9081_v2_revL_14Aug2019EnglishPromethion Download 10th June 2021 In the PromethION branch of the protocol, "Priming and loading the flow cell" step: - Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device - Added a note about correct insertion of the flow cell to avoid damaging the pins
June 10th, 2021DSS_9081_v2_revL_14Aug2019EnglishGridion Download 10th June 2021 In the PromethION branch of the protocol, "Priming and loading the flow cell" step: - Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device - Added a note about correct insertion of the flow cell to avoid damaging the pins
June 10th, 2021DSS_9081_v2_revL_14Aug2019EnglishMinion Download 10th June 2021 In the PromethION branch of the protocol, "Priming and loading the flow cell" step: - Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device - Added a note about correct insertion of the flow cell to avoid damaging the pins
January 20th, 2021DSS_9081_v2_revK_14Aug2019EnglishPromethion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021DSS_9081_v2_revK_14Aug2019EnglishGridion Download 9th Dec Compatibilities updated for EXP-WSH004
January 20th, 2021DSS_9081_v2_revK_14Aug2019EnglishMinion Download 9th Dec Compatibilities updated for EXP-WSH004
July 7th, 2020DSS_9081_v2_revJ_14Aug2019EnglishPromethion Download 07 July 2020 Updated equipment and consumables: we now recommend buying the NEBNext® Quick Ligation Reaction Buffer (NEB B6058) and T4 DNA Ligase 2M U/ml (NEB M0202) instead of the NEBNext Quick Ligation Module.
July 7th, 2020DSS_9081_v2_revJ_14Aug2019EnglishGridion Download 07 July 2020 Updated equipment and consumables: we now recommend buying the NEBNext® Quick Ligation Reaction Buffer (NEB B6058) and T4 DNA Ligase 2M U/ml (NEB M0202) instead of the NEBNext Quick Ligation Module.
July 7th, 2020DSS_9081_v2_revJ_14Aug2019EnglishMinion Download 07 July 2020 Updated equipment and consumables: we now recommend buying the NEBNext® Quick Ligation Reaction Buffer (NEB B6058) and T4 DNA Ligase 2M U/ml (NEB M0202) instead of the NEBNext Quick Ligation Module.
June 1st, 2020DSS_9081_v2_revI_14Aug2019EnglishPromethion Download 1st June Updated SQK-RNA002 kit to reflect cap colour changes
June 1st, 2020DSS_9081_v2_revI_14Aug2019EnglishGridion Download 1st June Updated SQK-RNA002 kit to reflect cap colour changes
June 1st, 2020DSS_9081_v2_revI_14Aug2019EnglishMinion Download 1st June Updated SQK-RNA002 kit to reflect cap colour changes
October 15th, 2019DSS_9081_v2_revH_14Aug2019EnglishPromethion Download 27th September 2019 Updated and expanded Troubleshooting guide
October 15th, 2019DSS_9081_v2_revH_14Aug2019EnglishGridion Download 27th September 2019 Updated and expanded Troubleshooting guide
October 15th, 2019DSS_9081_v2_revH_14Aug2019EnglishMinion Download 27th September 2019 Updated and expanded Troubleshooting guide
August 23rd, 2019DSS_9081_v2_revG_14Aug2019EnglishPromethion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019DSS_9081_v2_revG_14Aug2019EnglishGridion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 23rd, 2019DSS_9081_v2_revG_14Aug2019EnglishMinion Download 23rd August 2019 Troubleshooting guide added to the protocol, detailing the most common issues seen during a nanopore sequencing experiment.
August 14th, 2019DSS_9081_v2_revF_14Aug2019EnglishPromethion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 14th, 2019DSS_9081_v2_revF_14Aug2019EnglishGridion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 14th, 2019DSS_9081_v2_revF_14Aug2019EnglishMinion Download 14th August 2019 The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
August 2nd, 2019DSS_9081_v2_revE_22Nov2018English- Download 19th July 2019 Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
June 28th, 2019DSS_9081_v2_revD_22Nov2018English- Download Updated with new information for MinKNOW release v19.06: - Compression of queued reads - Pause functionality - UI improvements including: - Flow cell health at platform QC - Flow cell status on landing page - R10 flow cell running scripts - New Fast Models for RNA and DNA - PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files - Flongle hardware check for confirming Flongle adapter functions correctly
June 12th, 2019DSS_9081_v2_revC_22Nov2018English- Download Update to the flow cell priming section following the release of the Flow Cell Priming Kit (EXP-FLP002)
May 2nd, 2019DSS_9081_v2_revB_22Nov2018English- Download Updates for MinKNOW release 19.05
November 28th, 2018DSS_9081_v2_revA_22Nov2018English- Download -
November 23rd, 2018DSS_9067_v2_revA_22Nov2018English- Download -