25th May 2022
Have removed all references to poly(A)-tailed RNA use throughout the protocol, protocol now only references use of total RNA.
Snippet was already present in Overview directing user to correct protocol for sequencing poly(A)-tailed RNA.
25th May 2022
Have removed all references to poly(A)-tailed RNA use throughout the protocol, protocol now only references use of total RNA.
Snippet was already present in Overview directing user to correct protocol for sequencing poly(A)-tailed RNA.
25th May 2022
Have removed all references to poly(A)-tailed RNA use throughout the protocol, protocol now only references use of total RNA.
Snippet was already present in Overview directing user to correct protocol for sequencing poly(A)-tailed RNA.
23rd March 2022
- Updated input in the introduction of the protocol
- Added further information regarding RNA input in the equipment and consumables section
- Updated SPRI clean volume in the library prep
23rd March 2022
- Updated input in the introduction of the protocol
- Added further information regarding RNA input in the equipment and consumables section
- Updated SPRI clean volume in the library prep
23rd March 2022
- Updated input in the introduction of the protocol
- Added further information regarding RNA input in the equipment and consumables section
- Updated SPRI clean volume in the library prep
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
10th June 2021
In the PromethION branch of the protocol, "Priming and loading the flow cell" step:
- Added a note to wait 20 minutes after taking the flow cells out of the fridge, and wiping off any condensation before inserting the flow cells into the device
- Added a note about correct insertion of the flow cell to avoid damaging the pins
07 July 2020
Updated equipment and consumables: we now recommend buying the NEBNext® Quick Ligation Reaction Buffer (NEB B6058) and T4 DNA Ligase 2M U/ml (NEB M0202) instead of the NEBNext Quick Ligation Module.
07 July 2020
Updated equipment and consumables: we now recommend buying the NEBNext® Quick Ligation Reaction Buffer (NEB B6058) and T4 DNA Ligase 2M U/ml (NEB M0202) instead of the NEBNext Quick Ligation Module.
07 July 2020
Updated equipment and consumables: we now recommend buying the NEBNext® Quick Ligation Reaction Buffer (NEB B6058) and T4 DNA Ligase 2M U/ml (NEB M0202) instead of the NEBNext Quick Ligation Module.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
19th July 2019
Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
Updated with new information for MinKNOW release v19.06:
- Compression of queued reads
- Pause functionality
- UI improvements including:
- Flow cell health at platform QC
- Flow cell status on landing page
- R10 flow cell running scripts
- New Fast Models for RNA and DNA
- PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files
- Flongle hardware check for confirming Flongle adapter functions correctly