22nd June 2022
- Added a Sequence capture kit example
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
22nd June 2022
- Added a Sequence capture kit example
- Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
25th May 2022
In the 'Elution and amplification of DNA' page, updated the "PRM Adapters (10 μM) from the Ligation Sequencing Kit" to simply state "PRM Adapters (10 μM)".
In the 'Further amplification of low-yield samples (optional)' page, updated the "PRM Adapters (10 μM) from Nanopore Sequencing Kit" to simply state "PRM Adapters (10 μM)".
25th May 2022
In the 'Elution and amplification of DNA' page, updated the "PRM Adapters (10 μM) from the Ligation Sequencing Kit" to simply state "PRM Adapters (10 μM)".
In the 'Further amplification of low-yield samples (optional)' page, updated the "PRM Adapters (10 μM) from Nanopore Sequencing Kit" to simply state "PRM Adapters (10 μM)".
20th September 2019
- A note has been added at the end of the library preparation instructions about using the Sequencing Auxiliary Vials (EXP-AUX001) for splitting a library into two aliquots
20th September 2019
- A note has been added at the end of the library preparation instructions about using the Sequencing Auxiliary Vials (EXP-AUX001) for splitting a library into two aliquots
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
14th August 2019
The protocol has been expanded to include instructions for library prep on multiple nanopore sequencing devices. The user can select instructions specific to their device by selecting from the dropdown menu on the right hand side of the protocol.
19th July 2019
Protocol structure has been condensed to remove sections about DNA/RNA QC, software installation and use, and flow cell check. These sections are available as stand-alone documents, which are linked out from the protocol. The checklist protocol is now available for download from the button in the protocol header
Updated with new information for MinKNOW release v19.06:
- Compression of queued reads
- Pause functionality
- UI improvements including:
- Flow cell health at platform QC
- Flow cell status on landing page
- R10 flow cell running scripts
- New Fast Models for RNA and DNA
- PDF report and CSV files for Duty time and Cumulative output are now found in same location as actual reads and fastQ files
- Flongle hardware check for confirming Flongle adapter functions correctly
Update to the flow cell priming section, and instructions for topping up the flow cell with fuel, following the release of the Flow Cell Priming Kit (EXP-FLP002)
Updates to kit contents with the introduction of the Control Expansion kit (EXP-CTL001) and removal of the Lambda Control DNA from the Ligation Sequencing Kit