Main menu

DNA stability - storage


Note: the data displayed was generated using our legacy chemistry (SQK-LSK109), but the outcomes and recommendations are still applicable to our current chemistry.


We are in the process of investigating the stability of extracted genomic DNA over time when stored at different temperatures. DNA was extracted from a Bacillus subtilis cell pellet using the QIAGEN Genomic-tip procedure as outlined in the product details. The extracted DNA was eluted in TE buffer, aliquoted and stored at 4°C or -20°C. After set periods of time over twelve months, aliquots are removed from storage and libraries prepared for sequencing using our Ligation Sequencing Kit. Prior to sequencing, the samples were SPRI size-selected.

In Figure 1, data is presented on the effect of the different storage conditions on read N50. No impact on sequencing throughput was observed and all samples delivered 3-star performance: 8+ Gb in 48 hours on flow cell FLO-MIN106, equivalent to the Lambda DNA supplied with the Control Expansion pack (EXP-CTL001).

DNA stability storage Figure 1. The effect of storage of DNA at -20°C or at 4°C on the observed read N50 of DNA sequencing libraries. These data indicate some deterioration of read N50 may be observed when storing DNA at 4°C over a period of 6 months. Therefore, storage at -20°C is advised to maintain sample integrity.

Last updated: 6/25/2025

ドキュメントオプション

言語:

入門

MinION Starter Packを購入 ナノポア製品の販売 シークエンスサービスプロバイダー グローバルディストリビューター

ナノポア技術

ナノポアの最新ニュースを購読 リソースと発表文献 Nanopore Communityとは

Oxford Nanoporeについて

ニュース 会社沿革 持続可能性 経営陣 メディアリソース & お問い合わせ先 投資家向け パートナー向け Oxford Nanopore社で働く 現在の募集状況 営業上の情報 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Japanese flag