Ligation sequencing V14 - PCR barcoding (SQK-LSK114 with EXP-PBC001 or EXP-PBC096) (PBC_9182_v114_revM_30Jan2025)

DateVersionLanguageDeviceChanges
1/30/2025PBC_9182_v114_revM_30Jan2025EnglishGridION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025PBC_9182_v114_revM_30Jan2025EnglishMinION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025PBC_9182_v114_revM_30Jan2025EnglishPromethION
30th January 2025 In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024PBC_9182_v114_revL_12Dec2024EnglishGridION
12 December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024PBC_9182_v114_revL_12Dec2024EnglishMinION
12 December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024PBC_9182_v114_revL_12Dec2024EnglishPromethION
12 December 2024 - Addition of link to MinION Mk1D IT requirements in "Compatibility of this protocol". - Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell. - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024PBC_9182_v114_revK_07Mar2023EnglishGridION
21st August 2024 - Updated protocol introduction to new format. - Updated sample input recommendations to 100 ng for gDNA or amplicons. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024PBC_9182_v114_revK_07Mar2023EnglishMinION
21st August 2024 - Updated protocol introduction to new format. - Updated sample input recommendations to 100 ng for gDNA or amplicons. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024PBC_9182_v114_revK_07Mar2023EnglishPromethION
21st August 2024 - Updated protocol introduction to new format. - Updated sample input recommendations to 100 ng for gDNA or amplicons. - NEB reagent recommendations for purchase and use updated. - Updated the End-prep method in-line with new recommended reagents. - Updated the Adapter ligation step with new recommended method. - Updated library loading on flow cell recommendations with table for different DNA size.
August 21st, 2024PBC_9182_v114_revJ_07Mar2023EnglishPromethion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024PBC_9182_v114_revJ_07Mar2023EnglishGridion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
August 21st, 2024PBC_9182_v114_revJ_07Mar2023EnglishMinion Download 31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 31st, 2024PBC_9182_v114_revI_07Mar2023EnglishPromethion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 31st, 2024PBC_9182_v114_revI_07Mar2023EnglishGridion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
July 31st, 2024PBC_9182_v114_revI_07Mar2023EnglishMinion Download 07th March 2024 - Updated library loading recommendations to 35-50 fmols.
September 19th, 2023PBC_9182_v114_revG_07Mar2023EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated - Added amplicon tailed primer sequences to the introduction
September 19th, 2023PBC_9182_v114_revG_07Mar2023EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated - Added amplicon tailed primer sequences to the introduction
September 19th, 2023PBC_9182_v114_revG_07Mar2023EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated - Added amplicon tailed primer sequences to the introduction
May 3rd, 2023PBC_9182_v114_revF_07Mar2023EnglishPromethion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
May 3rd, 2023PBC_9182_v114_revF_07Mar2023EnglishGridion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
May 3rd, 2023PBC_9182_v114_revF_07Mar2023EnglishMinion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
April 28th, 2023PBC_9182_v114_revE_07Mar2023EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023PBC_9182_v114_revE_07Mar2023EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023PBC_9182_v114_revE_07Mar2023EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
March 24th, 2023PBC_9182_v114_revD_07Mar2023EnglishPromethion Download March 2023: - Updated how much DNA is expected and taken forwards from the first end-prep step
March 24th, 2023PBC_9182_v114_revD_07Mar2023EnglishGridion Download March 2023: - Updated how much DNA is expected and taken forwards from the first end-prep step
March 24th, 2023PBC_9182_v114_revD_07Mar2023EnglishMinion Download March 2023: - Updated how much DNA is expected and taken forwards from the first end-prep step
March 17th, 2023PBC_9182_v114_revC_07Mar2023EnglishPromethion Download March 2023: - Added instructions for preparing ONT reagents
March 17th, 2023PBC_9182_v114_revC_07Mar2023EnglishGridion Download March 2023: - Added instructions for preparing ONT reagents
March 17th, 2023PBC_9182_v114_revC_07Mar2023EnglishMinion Download March 2023: - Added instructions for preparing ONT reagents
March 16th, 2023PBC_9182_v114_revB_07Mar2023EnglishPromethion Download March 2023: - Updated input recommendations for gDNA in the "Equipment and consumables" section, as well as in the "End-prep" step.
March 16th, 2023PBC_9182_v114_revB_07Mar2023EnglishGridion Download March 2023: - Updated input recommendations for gDNA in the "Equipment and consumables" section, as well as in the "End-prep" step.
March 16th, 2023PBC_9182_v114_revB_07Mar2023EnglishMinion Download March 2023: - Updated input recommendations for gDNA in the "Equipment and consumables" section, as well as in the "End-prep" step.
March 8th, 2023PBC_9182_v114_revA_07Mar2023EnglishPromethion Download -
March 8th, 2023PBC_9182_v114_revA_07Mar2023EnglishGridion Download -
March 8th, 2023PBC_9182_v114_revA_07Mar2023EnglishMinion Download -