19th August 2025
- In 'Overview', library preparation time was increased to ~145 min to align with Table in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min.
- In section 2, 'Equipment and consumables', the tube format for the LSK114 kit was removed.
- In sections 3 and 5 'End-prep', step 5, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell' an important note was added to the bottom of this section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- In section 7, 'Priming and loading the MinION/PromethION Flow Cell', step 2, the instructions for using the single-use tube format for this kit was removed. And for the MinION and GridION branch, an important note was added to the bottom of section 7, stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
8/21/2025
LWP_9183_v114_revN_19Aug2025
English
MinION
19th August 2025
- In 'Overview', library preparation time was increased to ~145 min to align with Table in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min.
- In section 2, 'Equipment and consumables', the tube format for the LSK114 kit was removed.
- In sections 3 and 5 'End-prep', step 5, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell' an important note was added to the bottom of this section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- In section 7, 'Priming and loading the MinION/PromethION Flow Cell', step 2, the instructions for using the single-use tube format for this kit was removed. And for the MinION and GridION branch, an important note was added to the bottom of section 7, stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
8/21/2025
LWP_9183_v114_revN_19Aug2025
English
PromethION
19th August 2025
- In 'Overview', library preparation time was increased to ~145 min to align with Table in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min.
- In section 2, 'Equipment and consumables', the tube format for the LSK114 kit was removed.
- In sections 3 and 5 'End-prep', step 5, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell' an important note was added to the bottom of this section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- In section 7, 'Priming and loading the MinION/PromethION Flow Cell', step 2, the instructions for using the single-use tube format for this kit was removed. And for the MinION and GridION branch, an important note was added to the bottom of section 7, stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
8/19/2025
LWP_9183_v114_revN_19Aug2025
English
GridION
19th August 2025
- In 'Overview', library preparation time was increased to ~145 min to align with Table in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min.
- In section 2, 'Equipment and consumables', the tube format for the LSK114 kit was removed.
- In sections 3 and 5 'End-prep', step 5, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell' an important note was added to the bottom of this section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- In section 7, 'Priming and loading the MinION/PromethION Flow Cell', step 2, the instructions for using the single-use tube format for this kit was removed. And for the MinION and GridION branch, an important note was added to the bottom of section 7, stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
8/19/2025
LWP_9183_v114_revN_19Aug2025
English
MinION
19th August 2025
- In 'Overview', library preparation time was increased to ~145 min to align with Table in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min.
- In section 2, 'Equipment and consumables', the tube format for the LSK114 kit was removed.
- In sections 3 and 5 'End-prep', step 5, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell' an important note was added to the bottom of this section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- In section 7, 'Priming and loading the MinION/PromethION Flow Cell', step 2, the instructions for using the single-use tube format for this kit was removed. And for the MinION and GridION branch, an important note was added to the bottom of section 7, stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
8/19/2025
LWP_9183_v114_revN_19Aug2025
English
PromethION
19th August 2025
- In 'Overview', library preparation time was increased to ~145 min to align with Table in 'Steps in the sequencing workflow' where priming and loading of flow cell was increased from 5 min to 10 min.
- In section 2, 'Equipment and consumables', the tube format for the LSK114 kit was removed.
- In sections 3 and 5 'End-prep', step 5, the following was added to all device arms: 'Then cool down to between 4°C and 20°C on the thermal cycler or place the samples on ice.'.
- In section 7, 'Priming and loading the MinION and GridION Flow Cell' an important note was added to the bottom of this section stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- In section 7, 'Priming and loading the MinION/PromethION Flow Cell', step 2, the instructions for using the single-use tube format for this kit was removed. And for the MinION and GridION branch, an important note was added to the bottom of section 7, stating that when a flow cell is inserted into the MinION Mk1D and the lid is closed, there will be a small gap around the sides of the device.
- For the PromethION branch, the step for removing the PromethION Flow Cell out of the fridge was moved to the beginning of the section on 'Priming and loading the PromethION Flow Cell' to enable the flow cell to reach room temperature while the flow cell priming mix is prepared. And an optional action of performing a flow cell check was included.
- Section 8, 'Data acquisition and basecalling' was formatted to align with current protocols.
1/30/2025
LWP_9183_v114_revM_30Jan2025
English
GridION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
LWP_9183_v114_revM_30Jan2025
English
MinION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
1/30/2025
LWP_9183_v114_revM_30Jan2025
English
PromethION
30th January 2025
In "Adapter ligation and clean-up". the instructions for preparing the LFB or SFB have been changed to: "Thaw either Long Fragment Buffer (LFB) or Short Fragment Buffer (SFB) at room temperature and mix by vortexing. Then spin down and keep at room temperature."
12/12/2024
LWP_9183_v114_revL_12Dec2024
English
GridION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
LWP_9183_v114_revL_12Dec2024
English
MinION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/12/2024
LWP_9183_v114_revL_12Dec2024
English
PromethION
12 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
8/21/2024
LWP_9183_v114_revK_07Mar2023
English
GridION
21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024
LWP_9183_v114_revK_07Mar2023
English
MinION
21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
8/21/2024
LWP_9183_v114_revK_07Mar2023
English
PromethION
21st August 2024
- Updated protocol introduction to new format.
- Updated sample input recommendations to 100 ng for gDNA or amplicons.
- NEB reagent recommendations for purchase and use updated.
- Updated the End-prep method in-line with new recommended reagents.
- Updated the Adapter ligation step with new recommended method.
- Updated library loading on flow cell recommendations with table for different DNA size.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
31 July 2024
- Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase
07th March 2024
- Updated library loading recommendations to 35-50 fmols.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
19th September 2023
- Updated flow cell loading instructions to include light shield installation
- Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
1st August 2023
- Kit contents layout has been updated
- In priming and loading the flow cell, the flow cell priming mix instructions have been updated
