11th September 2025
- In 'Overview of the protocol', in the Table under 'Library preparation and sequencing', the timing for priming and loading a flow cell was increased from 5 min to 10 min.
- In 'Equipment and consumables', the image of the LSK114 kit in single-use tube format was removed. And instructions for the single-use tube format were removed from section 8, step 2.
- In section 4, 'DNA repair and end-prep', 'Check your flow cell' and section 6, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In section 4, 'DNA repair and end-prep', step 23, instructions were provided on how to resuspend the AMPure XP bead pellet with nuclease-free water.
- In section 6, 'Priming and loading the flow cell', the instruction for removing the flow cell out of the fridge was moved to the top of this section.
- In 'Troubleshooting', section 11, the video links for priming and loading a MinION Flow Cell were replaced with links for the PromethION Flow Cell.
