• 1.5 ml Eppendorf DNA LoBind tubes
• 0.2 ml thin-walled PCR tubes
• Nuclease-free water (e.g. ThermoFisher, cat # AM9937)

• Microfuge
• Vortex mixer
• Thermal cycler at 30° C and 80° C
• P1000 pipette and tips
• P100 pipette and tips
• P20 pipette and tips
• P10 pipette and tips
• P2 pipette and tips
• Timer

• Agilent Bioanalyzer (or equivalent)
• Qubit fluorometer (or equivalent for QC check)
• Eppendorf 5424 centrifuge (or equivalent)

Rapid Barcoding Kit

(optional consumables)

• Agencourt AMPure XP beads
• Freshly-prepared 70% ethanol in nuclease-free water
• 10 mM Tris-HCl pH 8.0 with 50 mM NaCl

Rapid PCR Barcoding Kit

• Agencourt AMPure XP beads
• Freshly prepared 70% ethanol in nuclease-free water
• LongAmp Taq 2X Master Mix (e.g. NEB M0287)
• 10 mM Tris-HCl pH 8.5 with 50 mM NaCl

16S Barcoding Kit

• Agencourt AMPure XP beads
• Freshly prepared 70% ethanol in nuclease-free water
• LongAmp Taq 2X Master Mix (e.g. NEB M0287)
• 10 mM Tris-HCl pH 8.0 with 50 mM NaCl

Rapid whole genome amplification

• Qiagen REPLI-g UltraFast Mini Kit
• Phosphate buffered saline (PBS)