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Secuenciación múltiple de metilación con representación reducida (RRMS) a partir de células mediante el kit SQK-NBD114.24 (RRMS_9209_v114_revI_21Oct2025)

DateVersionLanguageDeviceChanges
10/21/2025RRMS_9209_v114_revI_21Oct2025EnglishPromethION
21st October 2025 - In section 7, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 8, 9, and 11, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.24 kit) as it is no longer applicable. - In section 8, 'Adapter ligation and clean-up', 'Check your flow cell' and section 9, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. - In 'Troubleshooting', video links for the MinION Flow Cell loading were replaced with the PromethION Flow Cell loading.
10/21/2025RRMS_9209_v114_revI_21Oct2025EspañolPromethION
21st October 2025 - In section 7, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 8, 9, and 11, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.24 kit) as it is no longer applicable. - In section 8, 'Adapter ligation and clean-up', 'Check your flow cell' and section 9, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. - In 'Troubleshooting', video links for the MinION Flow Cell loading were replaced with the PromethION Flow Cell loading.
9/18/2025RRMS_9209_v114_revH_02Jul2025EnglishPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
9/18/2025RRMS_9209_v114_revH_02Jul2025EspañolPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
9/12/2025RRMS_9209_v114_revH_02Jul2025EnglishPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
9/12/2025RRMS_9209_v114_revH_02Jul2025EspañolPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
9/12/2025RRMS_9209_v114_revH_02Jul2025EspañolPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
7/8/2025RRMS_9209_v114_revH_02Jul2025EnglishPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
7/2/2025RRMS_9209_v114_revH_02Jul2025EnglishPromethION
02 July 2025 - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume. - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
6/13/2025RRMS_9209_v114_revG_09May2025EnglishPromethION
09 May 2025 - Protocol title update
4/29/2025RRMS_9209_v114_revF_29Apr2025EnglishPromethION
29 April 2025 - Updated "DNA fragmentation" and "DNA repair and end-prep" steps with correction to the expected DNA recovery and quantities taken forward into the library preparation
1/7/2025RRMS_9209_v114_revE_07Jan2025EnglishPromethION
07 January 2025 - Updated "DNA repair and end prep" incubation step to 20°C for 5 minutes, 65°C for 5 minutes. (was 20°C for 5 minutes, 60°C for 5 minutes)
1/7/2025RRMS_9209_v114_revE_13Dec2024EnglishPromethION
07 January 2025 - Updated "DNA repair and end prep" incubation step to 20°C for 5 minutes, 65°C for 5 minutes. (was 20°C for 5 minutes, 60°C for 5 minutes)
12/13/2024RRMS_9209_v114_revD_13Dec2024EnglishPromethION
13 December 2024 - Addition of MinION Mk1D device for RRMS in "Overview of the protocol". - Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells. - Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024RRMS_9209_v114_revC_20Nov2024EnglishPromethION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
8/20/2024RRMS_9209_v114_revB_31Jul2024EnglishPromethION
21 August 2024 - Updated downstream analysis section: recommended software versions. - Included demux step in downstream analysis section.
August 20th, 2024RRMS_9209_v114_revA_31Jul2024English- Download Protocol release

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