Rapid sequencing DNA - PCR Barcoding Kit 24 V14 (SQK-RPB114.24)

DateVersion numberLanguageDeviceChanges
Oct 16 2024RPB_9191_v114_revD_16Oct2024EnglishFlongle
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
Oct 16 2024RPB_9191_v114_revD_16Oct2024EnglishGridION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
Oct 16 2024RPB_9191_v114_revD_16Oct2024EnglishMinION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
Oct 16 2024RPB_9191_v114_revD_16Oct2024EnglishPromethION
16th October 2024 - Updated Introduction to the protocol to the slimmed down structure. - Updated reagent use from LongAmp Taq 2X Master Mix (e.g. NEB, cat # M0287) to newly recommended LongAmp Hot Start Taq 2X Master Mix (NEB, M0533).
Sep 17 2024RPB_9191_v114_revC_17Sep2024EnglishFlongle
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
Sep 17 2024RPB_9191_v114_revC_17Sep2024EnglishGridION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
Sep 17 2024RPB_9191_v114_revC_17Sep2024EnglishMinION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
Sep 17 2024RPB_9191_v114_revC_17Sep2024EnglishPromethION
17th September 2024 - Protocol checklist now available. - Included clarification in the PCR amplification conditions that 14 cycles is recommended as a starting point but can be adjusted depending on experiment needs.
Sep 19 2023RPB_9191_v114_revB_28Jun2023EnglishFlongle
19th September 2023 - Updated flow cell loading instructions to include light shield installation
Sep 19 2023RPB_9191_v114_revB_28Jun2023EnglishGridION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
Sep 19 2023RPB_9191_v114_revB_28Jun2023EnglishMinION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
Sep 19 2023RPB_9191_v114_revB_28Jun2023EnglishPromethION
19th September 2023 - Updated flow cell loading instructions to include light shield installation
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishFlongle Download
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishPromethion Download
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishGridion Download
September 19th, 2023RPB_9191_v114_revA_28Jun2023EnglishMinion Download