Sequence assembly is the process of matching reads that correspond to the same genomic region, to form larger contiguous sequence units.

Sequence assembly is most commonly used in the context of genome or transcriptome assembly.

There are a number of tools available for performing read assembly. For example, gDNA assembly can be achieved using tools such as Flye or Shasta.

Recommended best practices may vary depending on the species’ genome that you are trying to assemble, and we would recommend you read the assembly section of the Applications page to identify the current recommended best practices.

The EPI2ME product includes a workflow for the assembly of smaller (haploid) genomes – wf-bacterial-genomes. This may also be used for non-microbial samples.