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Dorado
High-performance, easy-to-use commandline basecaller for Nanopore data. Developer release for evaluation of new features. For information about the Dorado project, see https://github.com/nanoporetech/dorado.
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Dorado documentation
For more detailed advice, documentation and installation guides for DoradoVersion: 1.4.0
Release date: 2/19/2026
Approved use: For research use only
- RNA v5.3.0 basecalling models with improved coverage at 3' end of reads
- Add CRAM support using --emit-cram
- Updates and improvements to local haplotagging in Dorado variant
- Dorado correct now uses GPU if available on MacOS
- Update minimap2 build to use SSE4.1 optimisations
- Add basic support for TWIST-96B-UDI, TWIST-96C-UDI, and TWIST-96D-UDI barcodes
- Report the number of midstrand barcodes detected in Dorado demux output summary
- Fix crash in Dorado demux for BAMs without RG lines
- Restrict RG header lines to just the read groups that can be emitted to the target file
- Fix incorrect alignment genome in Dorado summary outputs when BAM file header contains multiple unordered SQ lines
- Other fixes and improvements
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