Nanopore sequencing of DNA concatemers reveals higher-order features of chromatin structure

Aditya Deshpande from Weill Cornell Medicine, USA talks about PORE-C a novel approach to characterising 3D chromatin conformation.
Current approaches are divided into chromosome conformation capture (Hi-C) based assays and complex ligation free methods.
PORE-C is a modification of the HI-C protocol, whereby short-read sequencing is replaced with Nanopore long-read sequencing methods. The concatemers sequenced can capture multiple interactions and concurrent DNA modification information lost using the methods discussed above, with a relatively simple protocol.
Aditya describes chromunity an algorithm for the detection of hubs of multiway contacts, the known conformation and frequency of certain genes and super loops within inactive regions of DNA.  This enables to conduct megabase scale phasing of complex rearrangements and chromosome-scale de novo assembly.