Flongle sequencing reveals cryptic amphibian diversity and builds local research capacity in the Ecuadorian Amazon

Abstract

Global biodiversity hotspots are under threat, and traditional sequencing methods are too inaccessible to adequately study mega-diverse ecological communities. To address this, we established a DNA barcoding laboratory at an educational research station in the Ecuadorian Amazon. With multiplexed amplicon sequencing on Flongle Flow Cells, we’ve uncovered cryptic diversity in amphibian communities, discovering several species new to science and informing conservation plans. Through practical, experiential-based courses and research experiences, we have capacitated 70+ Ecuadorian students and professionals in field-adapted molecular biology and nanopore sequencing techniques. As biodiversity hotspots continue to become threatened worldwide, we provide an example of an on-site, low-overhead nanopore sequencing laboratory that accelerates biodiversity research while capacitating local researchers to fight the biodiversity crisis.