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DAJIN-assisted multiplex genotyping to validate the outcomes of CRISPR-Cas-based genome editing


Genome editing induces various on-target mutations. Accurate identification of mutations in founder mice and cell clones is essential to perform reliable genome editing experiments. However, no genotyping method allows the comprehensive analysis of diverse mutations.

We developed a genotyping method with an on-target site analysis software named Determine Allele mutations and Judge Intended genotype by Nanopore sequencer (DAJIN) that can automatically identify and classify diverse mutations, including point mutations, deletions, inversions, and knock-in. Our genotyping method with DAJIN can handle approximately 100 samples within a day and may become a new standard for validating genome editing outcomes.

Authors: Akihiro Kuno, Yoshihisa Ikeda, Shinya Ayabe, Kanako Kato, Kotaro Sakamoto, Sayaka Suzuki, Kento Morimoto, Arata Wakimoto, Natsuki Mikami, Miyuki Ishida, Natsumi Iki, Yuko Hamada, Megumi Takemura, Yoko Daitoku,, Yoko Tanimoto, Tra Thi Huong Dinh, Kazuya Murata, Michito Hamada, Atsushi Yoshiki, Fumihiro Sugiyama, Satoru Takahashi, Seiya Mizuno

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