Main menu

Blake Billmyre: Using long-reads to tell one wtf from another wtf


Lightning talk: Blake Billmyre from Stowers Institute for Medical Research showcased his work characterising the WTF gene in the yeast Schizosaccharomyces pombe (fission yeast). He first described how alleles are evolutionary competitors. This is particularly true for the WTF gene, for which specific alleles enhance their own transmission to the next generation by producing a poison that kills offspring that do not contain that allele. WTF genes are part of a large and rapidly evolving family; however, Blake described that the repetitive nature of these genes and proximity to long terminal repeats makes the assembly of WTF loci particularly challenging when using traditional short-read sequencing technology. To overcome these issues, the team at Stowers are now using the long sequencing reads provided by nanopore technology to fully characterise these complex regions. Initial results have shown that copy number and sequence identity can vary greatly between strains, resulting in reproductive barriers between different strains. Concluding his talk, Blake stated that nanopore sequencing is allowing assembly of the WTF region and delineation of WTF genes with accuracy comparable to alternative sequencing approaches.

入门指南

购买 MinION 启动包 Nanopore 商城 测序服务提供商 全球代理商

纳米孔技术

订阅 Nanopore 更新 资源库及发表刊物 什么是 Nanopore 社区

关于 Oxford Nanopore

新闻 公司历程 可持续发展 领导团队 媒体资源和联系方式 投资者 合作者 在 Oxford Nanopore 工作 职位空缺 商业信息 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Chinese flag