Equipment and consumables for the Rapid Sequencing Kit family
Requirements
Equipment and consumables for the Rapid Sequencing Kit family
FOR RESEARCH USE ONLY
Contents
Consumables
Equipment
Optional Equipment
Additional materials for:
Consumables
- 1.5 ml Eppendorf DNA LoBind tubes
- 0.2 ml thin-walled PCR tubes
- Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
Equipment
- Microfuge
- Vortex mixer
- Thermal cycler at 30° C and 80° C
- P1000 pipette and tips
- P100 pipette and tips
- P20 pipette and tips
- P10 pipette and tips
- P2 pipette and tips
- Timer
Optional Equipment
- Agilent Bioanalyzer (or equivalent)
- Qubit fluorometer (or equivalent for QC check)
- Eppendorf 5424 centrifuge (or equivalent)
Additional materials for:
Rapid Barcoding Kit
(optional consumables)
- Agencourt AMPure XP beads
- Freshly-prepared 70% ethanol in nuclease-free water
- 10 mM Tris-HCl pH 8.0 with 50 mM NaCl
Rapid PCR Barcoding Kit
- Agencourt AMPure XP beads
- Freshly prepared 70% ethanol in nuclease-free water
- LongAmp Taq 2X Master Mix (e.g. NEB M0287)
- 10 mM Tris-HCl pH 8.5 with 50 mM NaCl
16S Barcoding Kit
- Agencourt AMPure XP beads
- Freshly prepared 70% ethanol in nuclease-free water
- LongAmp Taq 2X Master Mix (e.g. NEB M0287)
- 10 mM Tris-HCl pH 8.0 with 50 mM NaCl
Rapid whole genome amplification
- Qiagen REPLI-g UltraFast Mini Kit
- Phosphate buffered saline (PBS)