• Agencourt AMPure XP beads
• NEBNext® Companion Module for Oxford Nanopore Technologies® Ligation Sequencing (cat # E7180S). Alternatively, you can use the three NEBNext® products below:
• NEBNext FFPE Repair Mix (M6630)
• NEBNext End repair / dA-tailing Module (E7546)
• NEBNext Quick Ligation Module (E6056)
• 1.5 ml Eppendorf DNA LoBind tubes
• 0.2 ml thin-walled PCR tubes
• Nuclease-free water (e.g. ThermoFisher, cat # AM9937)
• Freshly prepared 70% ethanol in nuclease-free water

• Covaris g-TUBE (for fragmenting input amounts between 100 and 500 ng)

• Hula mixer (gentle rotator mixer)
• Magnetic rack
• Microfuge
• Vortex mixer
• Thermal cycler at 20° C and 65° C
• P1000 pipette and tips
• P200 pipette and tips
• P100 pipette and tips
• P20 pipette and tips
• P10 pipette and tips
• P2 pipette and tips
• Ice bucket with ice
• Timer

• Agilent Bioanalyzer (or equivalent)
• Qubit fluorometer (or equivalent for QC check)
• Eppendorf 5424 centrifuge (or equivalent)

PCR Sequencing Kit/PCR Barcoding Kit

• NEB Blunt/TA Ligase Master Mix (M0367)
• LongAmp Taq 2X Master Mix (e.g. NEB M0287)
• 10 mM Tris-HCl pH 8.5 with 50 mM NaCl
• Exonuclease I (NEB, M0293) - optional

1D sequencing selecting for long reads

• BluePippin device and gel cassette

PCR barcoding (with the 12 or 96 barcoding expansion)

• LongAmp Taq 2X Master Mix (e.g. NEB cat # M0287)
• Magnet for 96 well plates (for 96 barcodes)
• Multi-channel pipette (for 96 barcodes)
• 0.2 ml 96-well PCR plates and seals (for 96 barcodes)

Premium whole genome amplification

• Qiagen REPLI-g Midi Kit
• T7 Endonuclease I (NEB, cat # M0302)
• TE buffer: 10 mM Tris (pH 8.0), 0.1 mM EDTA
• PEG 8000, 50% w/v
• 0.5 M EDTA, pH 8
• 5 M NaCl (Sigma, cat # 71386)
• 1 M Tris-HCl pH 8.0