17th October 2025
- In 'Overview of the protocol', links for the EPI2ME amplicon workflow were updated. And in 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table.
- In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited.
- In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once.
- In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In 'Data acquisition and basecalling', screenshots for MinKNOW were removed.
- In 'Troubleshooting', sections on Voltrax and Kit 9 chemistry were removed as they are no longer supported.
10/17/2025
RAA_9198_v114_revM_17Oct2025
English
MinION
17th October 2025
- In 'Overview of the protocol', links for the EPI2ME amplicon workflow were updated. And in 'Library preparation', the text 'Adapter ligation' was updated to 'Rapid adapter attachment' to signify rapid chemistry and the timing for priming and loading a flow cell was increased from 5 min to 10 min in the Table.
- In 'Compatibility of this protocol', MinION Mk1B and Mk1C were removed, along with their links for IT requirements as these device documents have moved to legacy. This also includes removal of links to their user manuals in 'Data acquisition and basecalling'.
- In section 2, 'Equipment and consumables' text in the SQK-RBK114.96 kit image was edited.
- In section 3, 'Library preparation' , 'Check your flow cell' and section 4, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document. And in section 3, a note was added to use the barcode wells only once.
- In section 4, 'Priming and loading the MinION/GridION Flow Cell', a tip was added to bring the flow cell to room temperature for better visibility of the array. And the addition of BSA to the flow cell priming mix was changed from a 'recommended' to a 'required' instruction. A note was added to the end of this section to explain that when a flow cell is inserted into the MinION Mk1D, there will be a small gap around the sides of the device.
- In 'Data acquisition and basecalling', screenshots for MinKNOW were removed.
- In 'Troubleshooting', sections on Voltrax and Kit 9 chemistry were removed as they are no longer supported.
12/11/2024
RAA_9198_v114_revK_11Dec2024
English
GridION
11 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
12/11/2024
RAA_9198_v114_revK_11Dec2024
English
MinION
11 December 2024
- Addition of links to MinION Mk1D IT requirements in "Compatibility of this protocol" and user manual in "Data acquisition and basecalling", "How to start sequencing".
- Arrow which was previously at the bottom edge of the flow cell in Image 1b "Priming and loading MinION/GridION Flow Cell" was moved to the middle of the flow cell.
- Updated link for flow cell returns in "Flow cell reuse and returns" and removed note for flushing flow cells.
- Updated link for MinION temperature control in "Issues during a sequencing run", "Failed to reach target temperature".
11/15/2024
RAA_9198_v114_revJ_20Nov2024
English
GridION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
11/15/2024
RAA_9198_v114_revJ_20Nov2024
English
MinION
November 2024
- Updated kit content information and image to include new kit format for SQK-RBK114.24 with plated rapid barcodes.
9/30/2024
RAA_9198_v114_revI_30Sept2024
English
GridION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
9/30/2024
RAA_9198_v114_revI_30Sept2024
English
MinION
30th September 2024
- Moved protocol out of early access phase, removed early access wording.
4/29/2024
RAA_9198_v114_revH_29Nov2023
English
GridION
29 April 2024
- Updated references to EPI2ME (no longer called EPI2ME Labs) throughout document.
4/29/2024
RAA_9198_v114_revH_29Nov2023
English
MinION
29 April 2024
- Updated references to EPI2ME (no longer called EPI2ME Labs) throughout document.
02 April 2024
- Added note to 'Introduction to amplicon sequencing using the Rapid Barcoding Kit 24 or 96 V14' to specify correct primer design prior to amplicon generation.
- Added "Important" section to 'Equipment and Consumables' highlighting primer design and consideractions prior to amplcion generation.
02 April 2024
- Added note to 'Introduction to amplicon sequencing using the Rapid Barcoding Kit 24 or 96 V14' to specify correct primer design prior to amplicon generation.
- Added "Important" section to 'Equipment and Consumables' highlighting primer design and consideractions prior to amplcion generation.
January 2024
- Added wording throughout protocol to clarify each sample/barcode should be associated to a single species amplicon (this method is not suitable for mixed species amplicon samples).
January 2024
- Added wording throughout protocol to clarify each sample/barcode should be associated to a single species amplicon (this method is not suitable for mixed species amplicon samples).
