Human cfDNA multiplex sequencing from blood using SQK-NBD114.24 (CFM_9208_v114_revF_02Jul2025)

DateVersionLanguageDeviceChanges
7/2/2025CFM_9208_v114_revF_02Jul2025EnglishPromethION
02 July 2025 - Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table. - Added P2i as a compatible device in 'Compatibility of this protocol'. - Updated information for new kit format with increased Short Fragment Buffer (SFB) volume.
11/15/2024CFM_9208_v114_revD_20Nov2024EnglishPromethION
November 2024 - Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
10/10/2024CFM_9208_v114_revC_10Oct2024EnglishPromethION
10 October 2024 - Updated extraction method to elute sample in 23 µl (was 50 µl) - Secified expected yield to be ≥ 6 ng for 1 ml of plasma.
6/5/2024CFM_9208_v114_revB_15May2024EnglishPromethION
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June 4th, 2024CFM_9208_v114_revA_15May2024English- Download 2023年10月4日 - 更新实验清单中“接头连接和纯化”部分最后一步的格式。