8th October 2025
- In section 5, 'Native barcode ligation', an important note was included to only use the barcode wells once and in steps 9, 10 and 12, text was removed for the clear cap EDTA (a reagent in the previous version of the SQK-NBD114.24 kit) as it is no longer applicable.
- In section 6, 'Adapter ligation and clean-up', 'Check your flow cell' and section 7, 'Priming and loading the flow cell', 'Complete a flow cell check', the links for the flow cell check in the MinKNOW protocol were replaced with links for the flow cell check document.
- In 'Troubleshooting', video links for the MinION Flow Cell loading were replaced with links for the PromethION Flow Cell loading.
7/2/2025
CFM_9208_v114_revF_02Jul2025
English
PromethION
02 July 2025
- Increased the timing for priming and loading the flow cell from 5 min to 10 min in Library preparation Table.
- Added P2i as a compatible device in 'Compatibility of this protocol'.
- Updated information for new kit format with increased Short Fragment Buffer (SFB) volume.
11/15/2024
CFM_9208_v114_revD_20Nov2024
English
PromethION
November 2024
- Updated kit content information and image to include new kit format of SQK-NBD114.24 with plated native barcodes.
10/10/2024
CFM_9208_v114_revC_10Oct2024
English
PromethION
10 October 2024
- Updated extraction method to elute sample in 23 µl (was 50 µl)
- Secified expected yield to be ≥ 6 ng for 1 ml of plasma.
