Ligation sequencing V14 - Direct cDNA sequencing (SQK-LSK114)

DateVersion numberLanguageDeviceChanges
Jul 31 2024DCS_9187_v114_revI_31Jul2024EnglishGridION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 18th April 2024 - Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
Jul 31 2024DCS_9187_v114_revI_31Jul2024EnglishMinION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 18th April 2024 - Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
Jul 31 2024DCS_9187_v114_revI_31Jul2024EnglishPromethION
31 July 2024 - Changed reagent order addition in Library preparation/Adapter ligation and clean-up; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 18th April 2024 - Included information for the current incompatibility with the default set-up for wf-transcriptomes with this method.
March 12th, 2024DCS_9187_v114_revG_19Apr2023EnglishPromethion Download 07th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step - Updated library loading recommendations to 35-50 fmols.
March 12th, 2024DCS_9187_v114_revG_19Apr2023EnglishGridion Download 07th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step - Updated library loading recommendations to 35-50 fmols.
March 12th, 2024DCS_9187_v114_revG_19Apr2023EnglishMinion Download 07th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step - Updated library loading recommendations to 35-50 fmols.
March 7th, 2024DCS_9187_v114_revF_19Apr2023EnglishGridion Download 06th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
March 7th, 2024DCS_9187_v114_revF_19Apr2023EnglishMinion Download 06th March 2024 - Updated AMPure Beads (AXP) thaw and storage to be at room temperature in RNA degradation and second strand synthesis step
March 6th, 2024DCS_9187_v114_revE_19Apr2023EnglishPromethion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
March 6th, 2024DCS_9187_v114_revE_19Apr2023EnglishGridion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
March 6th, 2024DCS_9187_v114_revE_19Apr2023EnglishMinion Download 19th September 2023 - Updated flow cell loading instructions to include light shield installation - Updated flow cell loading recommendations in the "Adapter ligation and clean-up" step.
September 19th, 2023DCS_9187_v114_revD_19Apr2023EnglishPromethion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023DCS_9187_v114_revD_19Apr2023EnglishGridion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
September 19th, 2023DCS_9187_v114_revD_19Apr2023EnglishMinion Download 1st August 2023 - Kit contents layout has been updated - In priming and loading the flow cell, the flow cell priming mix instructions have been updated
August 1st, 2023DCS_9187_v114_revC_19Apr2023EnglishPromethion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
August 1st, 2023DCS_9187_v114_revC_19Apr2023EnglishGridion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
August 1st, 2023DCS_9187_v114_revC_19Apr2023EnglishMinion Download May 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps - This kit has moved to released phase.
April 28th, 2023DCS_9187_v114_revB_19Apr2023EnglishPromethion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023DCS_9187_v114_revB_19Apr2023EnglishGridion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 28th, 2023DCS_9187_v114_revB_19Apr2023EnglishMinion Download April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
April 19th, 2023DCS_9187_v114_revA_19Apr2023EnglishPromethion Download
April 19th, 2023DCS_9187_v114_revA_19Apr2023EnglishGridion Download
April 19th, 2023DCS_9187_v114_revA_19Apr2023EnglishMinion Download