PCR tiling of SARS-CoV-2 virus - Eco protocol (SQK-LSK109 with EXP-NBD104, EXP-NBD114 or EXP-NBD196) (PTCE_9122_v109_revR_10Feb2021)

DateVersionLanguageDeviceChanges
3/10/2023PTCE_9122_v109_revR_10Feb2021EnglishGridION
February 2023 - Updated product phase to 'Legacy'
3/10/2023PTCE_9122_v109_revR_10Feb2021EnglishMinION
February 2023 - Updated product phase to 'Legacy'
August 10th, 2022PTCE_9122_v109_revQ_10Feb2021EnglishMinion Download 1st July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
August 10th, 2022PTCE_9122_v109_revQ_10Feb2021EnglishGridion Download 1st July 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 30th, 2022PTCE_9122_v109_revP_10Feb2021EnglishGridion Download 30th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
June 30th, 2022PTCE_9122_v109_revP_10Feb2021EnglishMinion Download 30th June 2022 - Update the priming and loading the SpotON flow cells for both MinION and GridION with new diagrams.
April 13th, 2022PTCE_9122_v109_revO_10Feb2021EnglishGridion Download 14th April 2022 Protocol name change
April 13th, 2022PTCE_9122_v109_revO_10Feb2021EnglishMinion Download 14th April 2022 Protocol name change
October 11th, 2021PTCE_9122_v109_revN_10Feb2021EnglishGridion Download 11th Oct 2021 - Clarified flow cell loading recommendations
October 11th, 2021PTCE_9122_v109_revN_10Feb2021EnglishMinion Download 11th Oct 2021 - Clarified flow cell loading recommendations
September 13th, 2021PTCE_9122_v109_revM_10Feb2021EnglishGridion Download 13th Sept 2021 - Added tables outlining kit contents in the Equipment and Consumables section.
September 13th, 2021PTCE_9122_v109_revM_10Feb2021EnglishMinion Download 13th Sept 2021 - Added tables outlining kit contents in the Equipment and Consumables section.
August 6th, 2021PTCE_9122_v109_revL_10Feb2021EnglishGridion Download 20th July 2021 - Updated images in sections: 'Reverse transcription', 'PCR', 'End-prep' and 'Native Barcode Ligation' to clearly show the plates.
August 6th, 2021PTCE_9122_v109_revL_10Feb2021EnglishMinion Download 20th July 2021 - Updated images in sections: 'Reverse transcription', 'PCR', 'End-prep' and 'Native Barcode Ligation' to clearly show the plates.
July 15th, 2021PTCE_9122_v109_revK_10Feb2021EnglishGridion Download 15th July Updated the 'Equipment and consumables' section to remove the table with repeated barcode sequences
July 15th, 2021PTCE_9122_v109_revK_10Feb2021EnglishMinion Download 15th July Updated the 'Equipment and consumables' section to remove the table with repeated barcode sequences
June 17th, 2021PTCE_9122_v109_revJ_10Feb2021EnglishGridion Download 17th June - In 'Native barcode ligation', consumable Agencourt RNase-free XP beads have been replaced with Agencourt AMPure XP beads.
June 17th, 2021PTCE_9122_v109_revJ_10Feb2021EnglishMinion Download 17th June - In 'Native barcode ligation', consumable Agencourt RNase-free XP beads have been replaced with Agencourt AMPure XP beads.
June 16th, 2021PTCE_9122_v109_revI_10Feb2021EnglishGridion Download 16th June 2021 - Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.
June 16th, 2021PTCE_9122_v109_revI_10Feb2021EnglishMinion Download 16th June 2021 - Required settings in MinKNOW in the 'Sequencing and data analysis' section has been updated include instructions to toggle 'Override mid barcoding score' and to increase minimum mid barcoding score from 40 to 50.
April 28th, 2021PTCE_9122_v109_revH_10Feb2021EnglishGridion Download 28th April - Primer pool concentration in the change note table of 'Overview of the protocol' updated to the correct 10 µM for Josh Quick's protocol overview.
April 28th, 2021PTCE_9122_v109_revH_10Feb2021EnglishMinion Download 28th April - Primer pool concentration in the change note table of 'Overview of the protocol' updated to the correct 10 µM for Josh Quick's protocol overview.
April 28th, 2021PTCE_9122_v109_revG_10Feb2021EnglishGridion Download 28th April 2021 - Primer pool concentration in 'PCR' updated to the correct 100 µM. - Updated the change table in the 'Overview of the protocol' section to the correct volumes and concentrations for the PCR mastermix. - Added screenshots of MinKNOW for barcoding recommendations in 'Data acquisition and basecalling' section.
April 28th, 2021PTCE_9122_v109_revG_10Feb2021EnglishMinion Download 28th April 2021 - Primer pool concentration in 'PCR' updated to the correct 100 µM. - Updated the change table in the 'Overview of the protocol' section to the correct volumes and concentrations for the PCR mastermix. - Added screenshots of MinKNOW for barcoding recommendations in 'Data acquisition and basecalling' section.
April 21st, 2021PTCE_9122_v109_revF_10Feb2021EnglishGridion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
April 21st, 2021PTCE_9122_v109_revF_10Feb2021EnglishMinion Download 21st April 2021 Inclusion of the optional Adapter Mix II Expansion (EXP-AMII001) kit for instances when a user is running a subset of barcodes in one experiment and needs extra Adapter Mix II for more reactions: - EXP-AMII001 is included in the "Equipment and consumables" section - Explanation of when to use EXP-AMII001 in the "Adapter ligation and clean-up" step
March 24th, 2021PTCE_9122_v109_revE_10Feb2021EnglishGridion Download 24th March Updated protocol overview visible on the protocol library
March 24th, 2021PTCE_9122_v109_revE_10Feb2021EnglishMinion Download 24th March Updated protocol overview visible on the protocol library
March 24th, 2021PTCE_9122_v109_revD_10Feb2021EnglishGridion Download 18th March 2021 - "Downstream analysis and expected results" step: the recommended analysis pipeline and workflow packaging information has been updated. We have removed the recommendation to use RAMPART and have included the EPI2ME Labs Jupyter notebook tutorial and EPI2ME workflow.
March 24th, 2021PTCE_9122_v109_revD_10Feb2021EnglishMinion Download 18th March 2021 - "Downstream analysis and expected results" step: the recommended analysis pipeline and workflow packaging information has been updated. We have removed the recommendation to use RAMPART and have included the EPI2ME Labs Jupyter notebook tutorial and EPI2ME workflow.
February 24th, 2021PTCE_9122_v109_revC_10Feb2021EnglishGridion Download 17th Feb Updated the table in the 'Overview of the protocol': Eco PCR tiling of SARS-CoV-2 virus , step change: PCR updated to 6.3 ul from 3.8 ul.
February 24th, 2021PTCE_9122_v109_revC_10Feb2021EnglishMinion Download 17th Feb Updated the table in the 'Overview of the protocol': Eco PCR tiling of SARS-CoV-2 virus , step change: PCR updated to 6.3 ul from 3.8 ul.
February 17th, 2021PTCE_9122_v109_revC_10Feb2020EnglishGridion Download 17th Feb Updated the table in the 'Overview of the protocol': Eco PCR tiling of SARS-CoV-2 virus , step change: PCR updated to 6.3 ul from 3.8 ul.
February 17th, 2021PTCE_9122_v109_revC_10Feb2020EnglishMinion Download 17th Feb Updated the table in the 'Overview of the protocol': Eco PCR tiling of SARS-CoV-2 virus , step change: PCR updated to 6.3 ul from 3.8 ul.
February 11th, 2021PTCE_9122_v109_revB_10Feb2020EnglishGridion Download 11th Feb Updated the 'End-prep' step to transfer 5 ul of pooled PCR products to the dilution plate. Transfer 3.3 ul of dilution plate to end-prep plate.
February 11th, 2021PTCE_9122_v109_revB_10Feb2020EnglishMinion Download 11th Feb Updated the 'End-prep' step to transfer 5 ul of pooled PCR products to the dilution plate. Transfer 3.3 ul of dilution plate to end-prep plate.
February 10th, 2021PTCE_9122_v109_revA_10Feb2020EnglishGridion Download -
February 10th, 2021PTCE_9122_v109_revA_10Feb2020EnglishMinion Download -