Single-cell sequencing
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Ultra-rich single-cell transcriptome data without compromise
The analysis of transcriptomic heterogeneity at the single-cell level has provided new insights into many research areas, including cancer research, cell development and function, and immunology. However, the use of traditional short-read sequencing technology limits the ability to identify transcript abundance at the isoform level.
Oxford Nanopore reads of unrestricted length resolve this challenge, enabling the sequencing of full-length transcripts to gain a deeper understanding of complex biology. Oxford Nanopore provides an end-to-end solution for single-cell RNA sequencing — from library preparation in approximately three hours, through to intuitive single-cell analysis with EPI2ME.
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Single-cell transcriptomics workflow
Learn about the complete workflow for single-cell transcriptome analysis, starting from 10x Genomics cDNA, through to approximately three hour library prep and high-output sequencing on PromethION, and finishing with data analysis using EPI2ME.
Transcript isoform detection with single-cell resolution
Discover how, by combining 10x Genomics assays and nanopore reads of unrestricted length, researchers can measure differential gene expression of alternative transcript isoforms and cluster spatial expression data or cells by gene and isoform expression with single-cell resolution.
Single-cell Oxford Nanopore sequencing
Recommended device for single-cell sequencing
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PromethION 24
A flexible device for large-scale single-cell transcriptome sequencing on up to 24 independently addressable PromethION Flow Cells, providing scalability from low to high throughput. Leverage state-of-the-art algorithms and GPU technology with high-performance onboard compute.
