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Sequencing full-length transcripts for isoform-level expression analysis


Two-page image of the workflow

Overview

Accurate quantification and characterisation of isoform expression is important to investigate the mechanisms of diseases. Nanopore sequencing can span full-length transcripts in single reads, enabling comprehensive transcriptome characterisation at the isoform level, which simplifies transcriptome annotation to reveal novel transcripts and isoforms.

This end-to-end workflow provides a flexible method to sequence full-length transcripts for isoform-level expression analysis.

In this workflow overview, you will:

  • Find out how full-length transcript sequencing enhances bulk transcriptomics
  • Discover our best practice sequencing workflow in detail, starting from the recommended extraction method, through to primary analysis
  • Learn about our recommended sequencing kit and devices

入門

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