Understanding bacterial DNA methylation and analysis methods

How do you analyse DNA methylation from nanopore sequencing data?

Oxford Nanopore sequencing technology offers direct detection of DNA methylation by interpreting changes in the electrical signal as native DNA strands pass through the pore. This eliminates the need for chemical conversion or amplification, enabling a streamlined workflow for modification analysis.

In this webinar, we demonstrate an end-to-end bacterial methylation workflow, featuring:

• How the ATCC Genome Portal (AGP) provides high-quality assemblies, annotations, and methylation data

• Best practices for assembly and polishing to enhance modification detection

• Strategies to progress from assembly outputs and BED files to comparative motif analysis, using Helicobacter pylori datasets as an example

• Guidance on when to use v6 HAC (High Accuracy) or SUP (Super Accuracy) models in bacterial analysis workflows