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Systematic benchmarking of detection tools for CpG methylation from Nanopore sequencing


Existing methods for modified base detection using require either immunoprecipitation of DNA or chemical conversion like for example bisulfite treatment, lacking enough resolution and sensitivity. Nanopore sequencing can access native DNA at single-molecule resolution and detect base modifications from the Nanopore signal patterns. Despite the various detection tools available for modified bases from Nanopore sequencing, there has not been yet a systematic accuracy benchmarking to determine the strengths and limitations of these approaches.

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