Previous genome assemblies of Drosophilidae were restricted to species bred in laboratories, limiting our understanding of this model organism. The authors performed amplification-free nanopore sequencing of single wild flies, to expand the taxonomic diversity of Drosophilidae genomes. Despite the small sample volume of 35 ng DNA per fly, they successfully built 183 new genome assemblies for 179 species, at the affordable cost of US$150 per genome.
Authors: Bernard Y. Kim, Hannah R. Gellert, Samuel H. Church, Anton Suvorov, Sean S. Anderson, Olga Barmina, Sofia G. Beskid, Aaron A. Comeault, K. Nicole Crown, Sarah E. Diamond, Steve Dorus, Takako Fujichika, James A. Hemker, Jan Hrcek, Maaria Kankare, Toru Katoh, Karl N. Magnacca, Ryan A. Martin, Teruyuki Matsunaga, Matthew J. Medeiros, Danny E. Miller, Scott Pitnick, Michele Schiffer, Sara Simoni, Tessa E. Steenwinkel, Zeeshan A. Syed, Aya Takahashi, Kevin H-C. Wei, Tsuya Yokoyama, Michael B. Eisen, Artyom Kopp, Daniel Matute, Darren J. Obbard, Patrick M. O’Grady, Donald K. Price, Masanori J. Toda, Thomas Werner, Dmitri A. Petrov
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