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Sequencing and analysis of nanopore-only microbial isolates with the NO-MISS workflow


Whole-genome sequencing of microbial isolates provides valuable information for public health, clinical microbiology research, food safety, and microbial ecology. Nanopore sequencing provides distinct advantages when it comes to detection and characterisation of antimicrobial resistance, virulence genes, plasmids and other mobile genetic elements.

During this webinar we walked through the nanopore-only microbial isolate sequencing solution (NO-MISS): a flexible and rapid approach for whole-genome sequencing of bacterial isolates.

Viewers learnt:

  • How the rapid, end-to-end NO-MISS workflow enabled whole-genome characterisation of bacterial isolates.
  • How to extract nucleic acids, prepare libraries, and sequence microbial isolate research samples with the NO-MISS workflow.
  • How to analyse microbial isolate sequencing data with the EPI2ME platform.
Authors: Anna Maria Niewiadomska, Alex Trotter and Chris Alder

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