Main menu

Same-day, multiplexed, high-accuracy targeted liquid biopsy approach for low-tumor content treatment response monitoring


Motivation:

  • Pediatric brain tumor diagnostics can be difficult: Brain tumors make up a significant proportion of pediatric cancers. However, due to their sensitive location, many difficulties exist in providing effective diagnostics, treatments, and treatment response monitoring for these patients. MRIs can be difficult to interpret due to diffuse disease and radiation induced swelling can masquerade as tumor progression (pseudoprogression), further complicating interpretation.
  • Quantitative liquid-biopsy can be used to monitor treatment response: Prior work in our lab showed that same-day nanopore amplicon sequencing of cerebro-spinal fluid could aid in treatment response monitoring[1]. However, CSF is difficult to gather in pediatric patients — usually requiring a site visit and general anesthesia—increasing patient and family burden.

Research questions:

  • Proof-of-concept: Can a two-step amplification approach capture, amplify, and concatemerize multiple narrow cell-free DNA targets?
  • Speed: Can we perform same-day molecular diagnostics on cell-free DNA?
  • Accuracy: How does our diagnostic compare to gold-standard methods (e.g. ddPCR genotyping)?

Download the PDF

入門

MinION Starter Packを購入 ナノポア製品の販売 シークエンスサービスプロバイダー グローバルディストリビューター

ナノポア技術

ナノポアの最新ニュースを購読 リソースと発表文献 Nanopore Communityとは

Oxford Nanoporeについて

ニュース 会社沿革 持続可能性 経営陣 メディアリソース & お問い合わせ先 投資家向け パートナー向け Oxford Nanopore社で働く 現在の募集状況 営業上の情報 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Japanese flag