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Using the mouse 4T1 breast carcinoma cell line, we have investigated whether nuclear expression of mitochondrial pseudogenes might contribute to an apparent uneven pattern of mitochondrial expression by removing mitochondrial DNA from cells and carrying out strand-switch cDNA sequencing with the MinION. The current understanding is that the entire mitochondrial genome is transcribed in both directions, and these multi-gene transcripts are processed into individual genes, 13 of which are translated into proteins, and three long non-coding RNAs.