Restrander: rapid orientation and QC of long-read cDNA data
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When performing gene expression analysis on RNAsequencing data, preserving information about the direction of the RNA transcript is important, especially in areas of overlapping transcription. In direct-cDNA and PCR-cDNA protocols, either the first or secondstrand cDNAs may be sequenced, resulting in both forward and reverse reads that have to be ‘restranded’ bioinformatically. Here, we introduce Restrander, a lightning-fast, highly accurate and user-friendly tool for restranding cDNA sequencing data.