Real-time genomic pathogen, resistance, and host range characterisation from passive water sampling of wetland ecosystems

Wetlands are vital for global biodiversity but also hotspots for pathogen and antimicrobial resistance (AMR) transmission. Pathogen detection is challenging due to low microbial concentrations in water. Using Oxford Nanopore sequencing with passive water samplers, Perlas and Reska et al. successfully profiled wetland microbiomes, detected clinically relevant AMR genes, and identified potential hosts. This study demonstrates a cost-efficient, real-time approach that provides valuable insights into how the environment, wildlife, livestock, and human populations are intrinsically linked through microorganisms.

Sample type: microbial DNA, RNA and viral particles from water samples

Kit: Rapid Barcoding Kit, Ligation Sequencing Kit, Rapid PCR Barcoding Kit