DNA extraction from dried blood spots for Oxford Nanopore sequencing
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- Published on: March 9 2026
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This poster presents an optimised workflow for extracting high molecular-weight (HMW) DNA from dried blood spots (DBS) for whole-genome sequencing (WGS) and Adaptive Sampling (AS) using Oxford Nanopore technology. By evaluating multiple DBS card types and working with inputs below recommended levels, we demonstrate that DBS-derived DNA can deliver high-quality long-read data suitable for variant detection, methylation analysis, and targeted enrichment, even from limited input material.
Download this poster to discover:
- How a robust HMW DNA extraction protocol enables long-read nanopore WGS that is compatible with multiple different DBS card types, even from limited inputs
- How AS increases on-target coverage (80–100×) of a pharmacogenomic (PGx) panel while retaining genome-wide coverage (>10× outside targeted regions), enabling combined targeted and low-pass analyses
- A single-platform approach that enables comprehensive genomic and epigenomic analysis of DBS samples
