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Decoding the plant 3D genome architecture with Pore-C sequencing


Event abstract

Determining the 3D structure of the genome reveals spatial interactions between chromosomal regions. This crucial information can be used for genome scaffolding or for understanding how chromosomal architecture impacts gene expression. Pore-C is an end-to-end chromatin conformation capture workflow that uses the Oxford Nanopore platform to unravel 3D chromosome topology.

During this talk, New York University’s Dr. Jae Young Choi discussed how to perform Pore-C sequencing in plant species, to generate chromosome-level genome assemblies, and map chromatin interactions to identify candidate enhancer‒gene interactions.

Watch to learn:

  • How nanopore sequencing technology can be used for the sequencing and assembly of plant genomes
  • How to apply 3D chromosome conformation capture techniques to obtain chromosome-level genome assembly
  • How to use nanopore sequencing-based chromosomal contact data to infer chromatin regulation in genomes

Meet the speaker

Jae Young Choi is a postdoctoral researcher at New York University studying plant evolutionary genomics. Jae completed his Ph.D. at Cornell University in population genetics using Drosophila as a model system. His current research is focused on integrating functional genomics and population genetic theory to understand how plants have adapted to wild and human-made environments, ultimately leading to the diversification and speciation of lineages.

Authors: Jae Young Choi

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