Ninetails: a comprehensive tool for investigation of non-adenosine residues within poly(A) tails from direct RNA sequencing data
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Oxford Nanopore direct RNA sequencing (DRS) facilitated by the Ninetails package is the only method for the analysis of the composition of poly(A) tails, unaffected by reverse transcription and/or PCR amplification
Ninetails leverages a neural network trained for the detection of non-A residues within the poly(A) tails in DRS data
Ninetails can be used for visual inspection of DRS read signals