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NCM 2022: In-Silico enrichment of bacterial plasmids using nanopore adaptive sampling


Horizontal gene transfer via plasmids plays a major role regarding the spread of antibiotic resistance genes (ARGs). However, characterization of ARGs located on low abundant plasmids by whole genome sequencing is challenging. Although sample preparation methods can enrich the proportion of plasmid DNA before sequencing, these methods are expensive and laborious, and they might introduce a bias by enriching only for specific plasmid DNA sequences.

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