NanopoReaTA: a user-friendly tool for nanopore-seq real-time transcriptional analysis
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Oxford Nanopore Technologies’ (ONT) sequencing platform offers an excellent opportunity to perform real-time analysis during sequencing. This feature allows for early insights into experimental data and accelerates a potential decision-making process for further analysis. Here we introduce NanopoReaTA, a user-friendly real-time analysis toolbox for RNA sequencing data from ONT. Sequencing results from a running or finished experiment are processed through an R Shiny-based graphical user interface (GUI) with an integrated Nextflow pipeline for whole transcriptome and gene-specific analyses. NanopoReaTA provides visual snapshots of analysis results in progress, thus enabling interactive sequencing and rapid decision-making. The application can be installed via conda or docker and can be found on github: https://github.com/AnWiercze/NanopoReaTA.