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NA12878 Human Reference on Oxford Nanopore MinION

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  • shared.published_on: December 1 2016
  • shared.source: GitHub

We have sequenced the CEPH1463 (NA12878/GM12878, Ceph/Utah pedigree) human genome reference standard on the Oxford Nanopore MinION using 1D ligation kits (450 bp/s) using R9.4 chemistry (FLO-MIN106).

Human genomic DNA from GM12878 human cell line (Ceph/Utah pedigree) was either purchased from Coriell - "DNA" - (cat no NA12878) or extracted from the cultured cell line - "cells". As the DNA is native, modified bases will be preserved.

resources.authors: Mark Akeson, Andrew D Beggs, Thomas Nieto, Miten Jain, Matt Loose, Nicholas Loman, Sunir Malla, Justin O'Grady, Hugh E. Olsen, Josh Quick, Hollian Richardson, Jared T. Simpson, Terrance P. Snutch , Louise Tee, John R. Tyson

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