Identification of the causative agent is crucial for accurate antimicrobial therapy administration in infective endocarditis (IE).
Although positive culture remains the gold standard for microbiological identification, its sensitivity is limited after antibiotic administration. Thus, molecular characterization is useful to complement pathogen identification.
Long-read sequencing allows the analysis of the full-length 16S rRNA gene, in order to accurately identify bacteria at the species level.
In this sense, nanopore-based sequencing is fast, flexible and easy-to-use.
We aimed to validate long-read sequencing for rapid identification of bacterial species causing IE.
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