London Calling 2023: Zootails: implementing nanopore sequencing for veterinary diagnostics

We are developing methods using nanopore sequencing to rapidly identify the species and predict antimicrobial resistance in veterinary infections without the need for culture. We recently described the metagenomic approach we developed for use with canine urine and skin swab samples. Now, we are adapting this approach to different types of samples, from different animal species and types of infection. In this talk, I will describe the optimisation to date, including a strategy for reusing flow cells, testing of adaptive sampling, and our current analysis pipeline, as well as discussing our plans and considerations as we prepare to launch this protocol for cage-side, same-day use with veterinary patients in a university animal hospital.