A guide to transcriptomics with Oxford Nanopore

Overview

A deeper understanding of the diversity of RNA within a transcriptome is essential for the better study of biological mechanisms and cellular processes. Oxford Nanopore technology uniquely delivers high outputs of reads that span full-length transcripts, resulting in unambiguous isoform detection and comprehensive transcriptome characterisation. Nanopore reads enable differential isoform expression analysis, as well as allele-specific expression analysis. Additionally, nanopore sequencing can identify fusion transcripts that cannot be captured in full with short-read approaches.

This guide introduces cDNA and direct RNA nanopore sequencing, for ultra-rich transcriptomic data without compromise.

In this getting started guide, you will:

• Find out about the benefits of nanopore sequencing for bulk transcriptomics, and how it reveals isoform-level information missed by legacy short-read technologies

• Discover how to go from sample to answer with streamlined, end-to-end workflows for cDNA and direct RNA nanopore sequencing

• Find out about the range of PromethION sequencing devices

• Learn how nanopore transcriptomics data can be easily analysed using the EPI2ME data analysis workflow wf-transcriptomes

• Read real-world case studies describing how researchers are utilising cDNA and direct RNA nanopore sequencing to reveal hidden diversity in human health and disease