A guide to transcriptomics with Oxford Nanopore
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Overview
A deeper understanding of the diversity of RNA within a transcriptome is essential for the better study of biological mechanisms and cellular processes. Oxford Nanopore technology uniquely delivers high outputs of reads that span full-length transcripts, resulting in unambiguous isoform detection and comprehensive transcriptome characterisation. Nanopore reads enable differential isoform expression analysis, as well as allele-specific expression analysis. Additionally, nanopore sequencing can identify fusion transcripts that cannot be captured in full with short-read approaches.
This guide introduces cDNA and direct RNA nanopore sequencing, for ultra-rich transcriptomic data without compromise.
In this getting started guide, you will:
Find out about the benefits of nanopore sequencing for bulk transcriptomics, and how it reveals isoform-level information missed by legacy short-read technologies
Discover how to go from sample to answer with streamlined, end-to-end workflows for cDNA and direct RNA nanopore sequencing
Find out about the range of PromethION sequencing devices
Learn how nanopore transcriptomics data can be easily analysed using the EPI2ME data analysis workflow wf-transcriptomes
Read real-world case studies describing how researchers are utilising cDNA and direct RNA nanopore sequencing to reveal hidden diversity in human health and disease