Gapless assembly of maize chromosomes using long read technologies

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Creating gapless telomere-to-telomere assemblies of complex genomes is one of the ultimate challenges in genomics.

We used long read technologies and an optical map based approach to produce a maize genome assembly composed of only 63 contigs.

The B73-Ab10 genome includes gapless assemblies of chromosome 3 (236 Mb) and chromosome 9 (162 Mb), multiple highly repetitive centromeres and heterochromatic knobs, and 53 Mb of the Ab10 meiotic drive haplotype.

Authors: Jianing Liu, Arun S Seetharam, Kapeel Chougule, Shujun Ou, Kyle William Swentowsky, Jonathan Isaiah Gent, Victor Llaca, Margaret Woodhouse, Nancy Manchanda, Gernot G Presting, David Kudrna, Magdy Alabady, Candice Hirsch, Kevin Fengler, Doreen Ware, Todd Michael, Matthew Hufford, Kelly Dawe
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  • Published on: January 15 2020
  • Source: BioRxiv