Full-length transcriptomic analysis reveals osmoregulatory mechanisms in Coilia nasus eyes reared under hypotonic and hyperosmotic stress

In recent years, sea-level rise, caused by global warming, will trigger salinity changes. This will threaten the survival of aquatic animals. Till now, the osmoregulatory mechanism of Coilia nasus eyes has not been yet explored. Oxford Nanopore Technologies (ONT) sequencing was performed in C. nasus eyes during hypotonic and hyperosmotic stress for the first time.

22.5G clean reads and 26,884 full-length non-redundant sequences were generated via ONT sequencing. AS events, APA, TF, and LncRNA were identified. During hypotonic stress, 46 up-regulated DEGs and 28 down-regulated DEGs were identified. During hypertonic stress, 190 up-regulated DEGs and 182 down-regulated DEGs were identified. These DEGs were associated with immune, metabolism, and transport responses. The expression of these DEGs indicated that apoptosis and inflammation were triggered during hypotonic and hyperosmotic stress.

To resist hypotonic stress, polyamines metabolism and transport of Na⁺ and Cl⁻ from inter-cellular to extra-cellular were activated. During hyperosmotic stress, amino acids metabolism and transport of myo-inositol and Na⁺ from extra-cellular to inter-cellular were activated, while Cl⁻ transport was inhibited. Moreover, different transcript isoforms generated from the same gene performed different expression patterns during hypotonic and hypertonic stress.

These findings will be beneficial to understand osmoregulatory mechanism of C. nasus eyes, and can also improve our insights on the adaptation of aquatic animals to environmental changes.