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Detection and assembly of extrachromosomal DNA (ecDNA) in tumours


Perform rapid sequencing and methylation analysis of extrachromosomal DNA (ecDNA) using nanopore technology, to assess cancer heterogeneity, oncogene overexpression, and environmental adaptation potential.

Download the poster to discover:

  • The structure of ecDNA
  • A bioinformatics workflow for detecting and assembling ecDNAs, using whole-genome nanopore sequencing data
  • How real-time data streaming with nanopore sequencing technology enables the characterisation of ecDNA within the first few hours of sequencing
  • A multiplexed approach for analysing several tumour samples in one MinION sequencing run, followed by ecDNA assembly and methylation analysis

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