Long cDNA sequencing reads enable transcriptome analysis at isoform resolution

Accurate characterisation of transcript diversity requires sequencing approaches that provide sufficient read lengths to distinguish closely related isoforms. Oxford Nanopore sequencing delivers unrestricted read lengths that can span transcripts, preserving exon and fusion connectivity within individual molecules and enabling direct transcript structure observation.

In this application note, we present a comparison of RNA sequencing methods, demonstrating that sequencing libraries prepared with the updated protocol for the Oxford Nanopore cDNA-PCR Sequencing Kit produce longer reads and improved transcript coverage compared with the previous protocol and offer benefits over alternative RNA sequencing methods.

In this application note, you will:

• Discover the importance of long cDNA sequencing reads for confident isoform characterisation and transcript-level quantification
• Find out how the updated Oxford Nanopore cDNA-PCR protocol delivers longer, more complete cDNA reads than the previous protocol, Illumina RNA-seq, and PacBio Kinnex
• Find out about library prep with the cDNA-PCR Sequencing Kit and sequencing on PromethION