A guide to targeted sequencing with Oxford Nanopore
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Overview
Targeted sequencing enables regions of interest to be analysed in a cost-effective and fast workflow, which is beneficial for applications where whole-genome sequencing is inefficient or costly. Combining innovative enrichment methods with real-time sequencing, nanopore technology provides significant advantages over conventional targeted sequencing methodologies.
In this getting started guide, discover how targeted nanopore sequencing can be performed during a live sequencing run without PCR or additional library preparation, such as bisulfite conversion.
In this getting started guide, you will:
- Find out how adaptive sampling enhances targeted nanopore sequencing by enriching or depleting regions of interest
- Discover the benefits of nanopore sequencing
- Identify the appropriate nanopore sequencing kit for your experiment
- For any questions, see the FAQs for this technique
- Read real-world case studies of how researchers are utilising nanopore sequencing to perform targeted analysis and generate high-quality data, including enriching low-abundance bacterial species in metagenomic samples
