Main menu

DNA library stability - Ligation Sequencing Kit


Note: the data displayed was generated using our legacy chemistry (SQK-LSK109), but the outcomes and recommendations are still applicable to our current chemistry.

The final stage of DNA library preparation for the Ligation Sequencing Kit is to elute the library in Elution Buffer. Typically, after the elution step, the library is mixed with the sequencing buffer and loading beads, then immediately loaded onto the flow cell. However, users may want to store the library in Elution Buffer and load the flow cell at a later point in time.

To demonstrate the stability of the sequencing libraries produced using the Ligation Sequencing Kit, a large-scale library was generated using Lambda DNA as the template. The library was aliquoted into single-use tubes and stored under different conditons for various lengths of time.

No significant decrease in output or read length was observed when storing the libraries at -80°C, 4°C or 21°C over the course of the 12 week study. However, storage at -20°C for 12 weeks and 30°C for 1 week led to a decrease in output. Therefore, we recommend storing libraries in Eppendorf DNA LoBind tubes at 4°C for short-term or repeated use. For single use or longer-term storage of more than 3 months, we recommend storing at -80°C.

Lib stability lsk109 1

Figure 1. The output (Mb/ch/h) and read N50 values for libraries that have been stored at various temperatures for varying lengths of time. Sequencing libraries were generated using SQK-LSK109 and stored in Elution Buffer for varying lengths of time. After storage, the libraries were mixed with sequencing buffer and loading beads before loading onto a MinION flow cell for sequencing.

Version Changelog
v2, May 2025 Added wording to clarify results are applicable to newer kit chemistry
v1, August 2020 Initial protocol publication

Last updated: 6/25/2025

ドキュメントオプション

言語:

入門

MinION Starter Packを購入 ナノポア製品の販売 シークエンスサービスプロバイダー グローバルディストリビューター

ナノポア技術

ナノポアの最新ニュースを購読 リソースと発表文献 Nanopore Communityとは

Oxford Nanoporeについて

ニュース 会社沿革 持続可能性 経営陣 メディアリソース & お問い合わせ先 投資家向け パートナー向け Oxford Nanopore社で働く 現在の募集状況 営業上の情報 BSI 27001 accreditationBSI 90001 accreditationBSI mark of trust
Japanese flag