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Software release 2.1.0 for Dorado

Version: 2.1.0

Release date: 7/13/2026

Approved use: For research use only

Performance improvements for v6.0.0 HAC models on Blackwell GPUs

Performance of v5.0.0+ SUP models has also been improved, especially for short reads

Feature improvements and bug fixes for Dorado smallvar:

  • Improve the CPU efficiency of read splitting.
  • Support gVCF output from Dorado smallvar via the --gvcf option.
  • Correctly report variants in Dorado smallvar when the reference is lower case.
  • Sort and deduplicate processed_regions.bed file in Dorado smallvar.
  • Add --hemizygous-regions option to Dorado smallvar, to specify regions in which haploid variant calls are expected.
  • Remove duplicated fileformat header line from VCF output.
  • Bug fix for overlapping simple variants by removing redundant allele coverage check in Dorado smallvar.
  • Fix output of overlapping variants due to trimming of processed regions before merging.

Other updates and improvements:

  • Add --MD, --cs and --rmq to the list of supported minimap options.
  • No longer create a .fai index file on disk alongside alignment references.
  • Fix sp:i tag to contain the correct split points in all cases when a read has been split multiple times.
  • Add support for single-ended dual barcode arrangements (two barcodes at one end of a read) and arrangements with split inter-barcode flanking regions.
  • Update to minimap2 v2.31.
  • Pin POD5 version to 0.3.39.
  • Fix batch size benchmarking while running LSTM models with variable chunk sizes.

Last updated: 7/13/2026

Latest version: 2.1.0

Approved use: For research use only

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