Date	Version	Language	Device		Changes
9/9/2024	T2T_9179_v114_revL_31Jul2024	English	PromethION		09 September 2024 - Moved protocol into legacy phase. - Links to new telomere-to-telomere protocol attached.
7/31/2024	T2T_9179_v114_revK_31Jul2024	English	PromethION		31 July 2024 - Changed reagent order addition in 'Day 1: Duplex experiment' and 'Day 4: Pore C expt'; Ligation Adapter (LA) added before Ligation Buffer (LB) and NEBNext Quick T4 Ligase 07 February 2024 All changes applied to the __Ultra-long DNA experiment__ in line with our SQK-ULK114 protocol updates (also implemented 07 Feb 2024). - Updated the Clean-up stage of the protocol to new method NOT using the Precipitation Star (PS) - Updated Flow Cell loading instructions specific to ULK libraries <br> Additional updates to Flow cell washing and reloading in line with our SQK-WSH004 and SQK-WSH004-XL updates. - Method updated to split the addition of the Flow Cell wash mix into two steps of 200 µl, with a 5 minute pause in-between. - Updated method with more extensive instructions on use and handling.
February 27th, 2024	T2T_9179_v114_revJ_09Feb2023	English	-	Download	07 February 2024 All changes applied to the __Ultra-long DNA experiment__ in line with our SQK-ULK114 protocol updates (also implemented 07 Feb 2024). - Updated the Clean-up stage of the protocol to new method NOT using the Precipitation Star (PS) - Updated Flow Cell loading instructions specific to ULK libraries <br> Additional updates to Flow cell washing and reloading in line with our SQK-WSH004 and SQK-WSH004-XL updates. - Method updated to split the addition of the Flow Cell wash mix into two steps of 200 µl, with a 5 minute pause in-between. - Updated method with more extensive instructions on use and handling.
February 8th, 2024	T2T_9179_v114_revI_09Feb2023	English	-	Download	07 February 2024 All changes applied to the __Ultra-long DNA experiment__ in line with our SQK-ULK114 protocol updates (also implemented 07 Feb 2024). - Updated the Clean-up stage of the protocol to new method NOT using the Precipitation Star (PS) - Updated Flow Cell loading instructions specific to ULK libraries <br> Additional updates to Flow cell washing and reloading in line with our SQK-WSH004 and SQK-WSH004-XL updates. - Method updated to split the addition of the Flow Cell wash mix into two steps of 200 µl, with a 5 minute pause in-between. - Updated method with more extensive instructions on use and handling.
September 19th, 2023	T2T_9179_v114_revG_09Feb2023	English	-	Download	4th July 2023 - Updated all wash steps to include recommendation to remove waste after hour incubation with nuclease wash.
July 21st, 2023	T2T_9179_v114_revF_09Feb2023	English	-	Download	4th July 2023 - Updated all wash steps to include an extra recommendation to remove waste buffer between flow cell priming mix flushes after a nuclease wash.
June 15th, 2023	T2T_9179_v114_revE_09Feb2023	English	-	Download	7th June 2023 - Updated duplex basecalling recommendations to use Dorado and corrected MinKNOW sequencing parameter recommendations. - Included an image of an example of what can be expected to see in a pore scan on all the wash step pages.
April 24th, 2023	T2T_9179_v114_revD_09Feb2023	English	-	Download	April 2023 - Updated duplex basecalling recommendations to use Dorado
April 24th, 2023	T2T_9179_v114_revC_09Feb2023	English	-	Download	April 2023 - Added flow cell priming mix volumes to the "Priming and loading" steps
March 9th, 2023	T2T_9179_v114_revB_09Feb2023	English	-	Download	February 2023 - Updated the "Duplex experiment: Data acquisition and basecalling" to recommend using Fast basecalling and have added further information for duplex basecalling on Guppy. - Updated the introduction to reference the UCSC Nanopore Production Center
February 9th, 2023	T2T_9179_v114_revA_09Feb2023	English	-	Download	-